Fig. 5

miR- 18a- 5p downregulation partly reversed the effects of miR- 18a- 5p silencing on LPS-induced cell damage in THP- 1 cells. A Changes in miR- 18a- 5p levels in THP- 1 cells after 48 h of induction with various LPS concentrations. B miR- 18a- 5p levels vary in THP- 1 cells induced by 1 µg/ml LPS for different durations. Transfection with miR- 18a- 5p inhibitor alone (C) or LPS-induced silencing of CYP1B1-AS1 alters THP- 1 cells (D). E-F Flow cytometry and ELISA assay were used to assess cell apoptosis, and inflammation factors in LPS, si-CYP1B1-AS1, and miR- 18a- 5p inhibitor CO-treated cells. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001