Skip to main content
Fig. 4 | BMC Immunology

Fig. 4

From: LncRNA CYP1B1-AS1 as a clinical biomarker exacerbates sepsis inflammatory response via targeting miR- 18a- 5p

Fig. 4

CYP1B1-AS1 directly interacts with miR- 18a- 5p. A Investigation of CYP1B1-AS1 subcellular localization in THP- 1 cells. B Venn diagram presented the overlapping target miRNAs of CYP1B1-AS1 predicted by LncBooK2 and DIANA databases. C The putative binding sequences were presented. The targeting relationship between CYP1B1-AS1 and miR- 18a- 5p was validated using Dual-luciferase report assay (D), RIP assay (E), and RNA pull-down assay (F). G The serum miR- 18a- 5p in the subjects was detected. H Pearson coefficient was conducted to examine the correlation between serum miR- 18a- 5p and CYP1B1-AS1 levels in patients with sepsis. I The mRNA levels of miR- 18a- 5p were altered in THP- 1 cells that were induced with LPS and transfected with si-CYP1B1-AS1. ** P < 0.01, *** P < 0.001, **** P < 0.0001

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us