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Table 1 Comparison between phenograph clusters and manually gated cell phenotypes

From: Integrated bulk RNA sequencing and mass cytometry analysis reveal the circulating immune landscape in ischemic and hemorrhagic Moyamoya disease

Cell Lineages

PhenoGraph Clusters

Cell Phenotypes

T cells

T cells

CD3+

 

CD4+T cells

CD4+

 

CD8+T cells

CD8+

 

DPT cells

CD4+CD8+

 

DNT cells

CD4−CD8−

 

NKT cells

CD56+

 

Treg cells

CD4+CD25+

 

γδT cells

TCRγδ+

B cells

B cells

CD19+

 

B01

CD45RAlow CD27−

 

B02

CD27+

 

B03

CD27high

 

B04

CCR7+

 

B05

PD-1+

NK cells

NK cells

CD56+

 

NK01

CD45RAhigh HLA-DR−

 

NK02

CD56high

 

NK03

CD45RAhigh HLA-DR+

 

NK04

HLA-DRhigh

 

NK05

PD-1+

 

NK06

CCR7high

 

NK07

CXCR4high

Monocytes

Monocytes

CD14+

 

M01

CD45ROhigh

 

M02

CD45ROlow CD45RAhigh

 

M03

HLA-DRhigh

 

M04

CD45ROlow CD45RAlow HLA-DRlow

 

M05

PD-1+

 

M06

CCR7+

DCs

DCs

CD3−CD19−CD56−CD14−

 

DC01

HLA-DRhigh

 

DC02

CD11b+CD45RAhigh CD45ROhigh

 

DC03

CD11b+CD45RAhigh CD45ROlow

 

DC04

CD11b+CD45RAlow CD45ROhigh

 

DC05

CD11b−CCR6−CXCR4−CD45RAlow

 

DC06

CXCR4high

 

DC07

CCR6+

  1. DCs indicate dendritic cells; DNT, double-negative T; DPT, double-positive T; and NK, natural killer