Fig. 5

In vitro B lymphopoiesis of HSCs, LMPPs, CLP in the 1%BSA X-VIVO15 complete medium. (A)The development of B-lineage cells was analyzed via flow cytometry with antibodies against CD11b and CD19 during cultured for 7 d. (B) The increase in the number of CD19 + and CD11b + cells under culture. The percentage mean each cell accounted for the total cell frequency. **P<0.01. ***P<0.001. ****P<0.0001