Fig. 3

Comparison of B-cell production from CLP in the presence or absence of the indicated cytokines(s) supplementation. (A) Microscopic observation of B lymphopoiesis of CLP in vitro. (B) Flow cytometry analysis of in vitro B lymphopoiesis in culture (Gated on live cells). The total cells derived from culture in the absence of cytokine supplementation (PBS), IL-7 and FLT3L or SCF, FLT3L and SCF, IL-7, FLT3L and SCF for 7 d were analyzed via flow cytometry with antibodies against B220, CD19. (C) The total cells were analyzed by flow cytometry in the media. The absolute number and percentage of B220 + CD19 + cells under the different culture media. NS, not significant. The percentage means the cell frequency of CD19 + B220 + cells among total cells. *P<0.05. **P<0.01. ***P<0.001. ****P<0.0001