Fig. 2

Expansion of in vitro CLP cells in X-VIVO15 system containing different concentrations of FBS and BSA on day 7. (A) Morphology of in vitro mouse CLP in 1%BSA X-VIVO15, 1%FBS X-VIVO15, 5%FBS X-VIVO15, 10%FBS X-VIVO15 and 20%FBS X-VIVO15 on day 7. B scale bars indicate 750 μm. (B) The total numbers of cells and CD19 + B220 + cells obtained in the 5 medium systems on day 7. The percentage means the cell frequency of CD19 + B220 + cells among total cells. *P<0.05. **P<0.01. ***P<0.001