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Fig. 1 | BMC Immunology

Fig. 1

From: In vitro differentiation of common lymphoid progenitor cells into B cell using stromal cell free culture system

Fig. 1

Expansion of in vitro CLP cells in X-VIVO15, RPMI-1640, IMDM, α-MEM and DMEM on day 7. (A) Gating strategy for the isolation of CLP (Lineage-CD127 + CD117low Sca-1low CD135+) from bone marrow on the MoFlo Astri os EQ. (B) Morphology of in vitro mouse CLP in 5 medium systems on day 7. B scale bars indicate 750 μm. (C) The total numbers of cells and CD19 + B220 + cells obtained in the 5 medium systems on day 7. The percentage means the cell frequency of CD19 + B220 + cells among total cells. **P<0.01. ***P<0.001

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BMC Immunology

ISSN: 1471-2172

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