Fig. 1

Analysis of Ves v 5 expression in BL21 (DE3) using SDS‒PAGE 12.5%. A: Lanes 1 and 2: bacterial protein before and after induction with IPTG, respectively; Lane 3: bacterial pellet after induction with IPTG; Lane 4: pre-stained protein marker. B: Lane 1: protein marker; lanes 2–6: 5 eluates from the Ni-NTA column