Table 1 Quantitative real time PCR primers
Primer Name | Primer sequence |
|---|---|
miRNA-34a stem-loop (RT) | GTCGTATCCAGTGCAGGGTCCGAGGTATT CGCACTGGATACGACACAACC |
U6 specific (RT) | GAATTTGCGTGTCATCCTTG |
GAPDH | F: CATGAGAAGTATGACAACAGCCT R: AGTCCTTCCACGATACCAAAGT |
PD-L1 | F: GGTGGTGCCGACTACAAGCGA R: TGACTTCGGCCTTGGGGTAGC |
Ī±SMA | F: TGGCTATTCCTTCGTTACTACTGCT R: CATCAGGCAACTCGTAACTCTTCTC |
EPCAM | F: AATCGTCAATGCCAGTGTACTT R: TCTCATCGCAGTCAGGATCATAA |
U6 | F: GCTTCGGCAGCACATATACTAAAAT R: CGCTTCACGAATTTGCGTGTCAT |
miRNA-34a | F: GGGATGGCAGTGTCTTAGC Universal R: GTGCAGGGTCCGAGGT |