Fig. 4

Multifactor regulatory network of DE-NiNRGs and identification of necroinflammation and necroptosis related subtypes in patients with MMD. A: mRNA-miRNA regulatory network of DE-NiNRGs. Pink diamonds: DE-NiNRGs. Green polygons: miRNAs. B: Unsupervised clustering result of MMD samples in training set, k = 2. Darker color in the consensus matrix represented higher possibility that two samples belonged to the same cluster. C: CDF (Cumulative Distribution Function) curves of different k value in clustering. D: PCA plot of 2 MMD subtypes. Red and green dots represented samples belonged to the different clusters. E: Curve of the change of area under CDF curves. F: Tracking plot of unsupervised clustering of MMD samples with different k values. The horizontal axis represents different samples, and different colors represent the different clusters to which each sample belonged under different k value on the vertical axis. G: Box plot of immune infiltration score. Blue: MMD cluster (1) Red: MMD cluster (2) Horizontal axis: 28 kinds of immune cells. Vertical axis: Immune infiltration score. ‘*’: P value between 0.05 and 0.01. ‘**’: P value < 0.01