Fig. 5

The counteractive effect of SIRT1 on NF-κB signaling by TAMI-M in vivo. A Immunoblotting analysis of SIRT1, AR, and CD36 expression in control, DHT-induced AGA mice, and TAMI-M treated mice (2 × 0.6 mg/kg, subcutaneously). B Expression of inflammatory markers: IL-6, P-STAT3, and P-NF-κB in the same groups. C Expression of TNF-α, HSP70, and NLRP3 in the control, DHT-induced AGA, and TAMI-M treated groups. Relative protein expression levels were quantified and normalized to β-actin. Individual data points are shown to illustrate variation within groups. Statistical significance was determined using one-way ANOVA followed by post hoc comparisons (Tukey’s test). Significant differences are indicated as *p < 0.05, **p < 0.01, and ***p < 0.001