Fig. 3

Effect of TAMI-M on Poly I: C-induced production of proinflammatory chemokines, SIRT1, and Caspase-1 in HORS: A HORS cells were treated with Poly I: C for 3 h to simulate an inflammatory environment resembling alopecia areata pathology. TAMI-M was applied to evaluate its potential effects. Relative mRNA expression levels of proinflammatory chemokines were quantified using qPCR. Treatment with TAMI-M significantly reduced chemokine production compared to Poly I: C-induced controls. B TAMI-M treatment restored SIRT1 expression levels, which were downregulated following Poly I: C induction. C Caspase-1 activity, a marker of inflammasome activation, was measured by qPCR. TAMI-M treatment significantly reduced Caspase-1 activity in HORS cells compared to Poly I: C-treated controls. Statistical significance was determined using one-way ANOVA followed by post hoc comparisons (Tukey’s test). Significant differences are denoted as *p < 0.05, **p < 0.01, and ***p < 0.001 between groups