Fig. 2

Effect of TAMI-M treatment on proinflammatory chemokines, and cytokines in C3H/HeJ AA model mice: A C3H/HeJ mice were induced with AA-like symptoms using poly I: C and IFN-γ, mimicking the inflammatory milieu observed in human alopecia areata. TAMI-M was administered subcutaneously at a dose of 0.8 mg/kg weekly or monthly. B Relative expression levels of proinflammatory chemokines and cytokines were measured in dorsal skin samples via qPCR following TAMI-M treatment. Data reflect significant reductions in inflammatory markers in treated groups compared to vehicle controls. C miR-485-3p expression levels were evaluated in the skin of treated and control mice. Weekly and monthly TAMI-M dosing regimens both resulted in substantial downregulation of miR-485-3p expression compared to vehicle controls. Statistical significance was determined using one-way ANOVA followed by post hoc comparisons (Tukey’s test). Statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle (before poly I: C induction); *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle (after poly I: C induction)