Fig. 1

Efficacy of TAMI-M weekly and monthly treatments in C3H/HeJ AA model Mice: A Schematic representation of the experimental design. Fifteen-week-old C3H/HeJ mice were injected subcutaneously with poly I: C and IFN-γ to induce AA-like symptoms. TAMI-M was administered subcutaneously at a dose of 0.8 mg/kg either weekly or monthly, while control animals received PBS (vehicle). Each treatment group consisted of 5 mice (n = 5). B Representative images of hair regrowth and alopecia severity were taken at defined intervals post-treatment on 8, 9 and 11 weeks. Images show the extent of lesional area before and after treatment in the TAMI-M weekly, monthly, and vehicle groups. C Quantitative analysis of AA lesion areas in the dorsal skin of treated and control mice was performed. Statistical significance was determined using one-way ANOVA followed by post hoc comparisons (Tukey’s test). The effects of TAMI-M treatment against AA lesion formation were significant for both dosing regimens in the presence of poly I: C and IFN-γ treatment. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle (before poly I: C induction); *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle (after poly I: C induction)