Fig. 6

In vivo hypoxia-PAH model was established to validate the cell chat between GPNMB⁺ macrophages and CD44⁺ SMCs. A Schematic diagram of the animal experimental design of PAH. B Representative images of H&E staining among groups. Scale bar = 20 μm. C Representative images of Masson staining among groups. Scale bar = 20 μm. D Representative images of GPNMB (purple), F4/80 (pink), α-SMA (red) and CD44 (pink) mmunofluorescent staining. Scale bar = 20 μm. The significance of difference between the means of groups was evaluated using one-way ANOVA; n = 6 per group