Fig. 6
From: β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1
β-Glucan regulates immune response of B cells through Dectin-1 directly (A) Dectin-1 expression on wild type mice splenic B cells. Gating strategy and representative dot plots. Data shown was gated on the CD19-positive and CD3-negative B cells (red) population. (B) Wild type mice or Dectin-1−/− mice splenic cells were purified mouse primary B cells. Cells were stimulated with LPS, with or without WGP in vitro for 72 h. Single cell suspensions were stained with fluorescein-labeled mAbs. The summarized data are shown for B cells (B220lo CD138+). (C-D) Dectin-1−/− mice bone marrow-derived DC co-cultured with B cells, which purified from wild type mice or Dectin-1−/− mice splenic cells. Cells were stimulated with LPS, with or without WGP in vitro for 3 days. Single cell suspensions were stained with fluorescein-labeled mAbs. The summarized data are shown for immunosuppressive B cells (CD11b+ CD19+) (C), (CD11b+ B220lo CD138+) (D). A representative histogram from three independent experiments with similar results is shown. (• LPS, ▪ LPS + WGP, * P < 0.05, **P < 0.01, ***P < 0.001)