Fig. 5
From: β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1
β-Glucan regulates immune response of B cells co-cultured with antigen presenting cells We separated the cells into 5 groups, including wild type mice splenic total cells (TC), depleted mouse primary CD4+ T splenic cells (-CD4+ T), purified mouse primary B cells (B), purified mouse primary B cells co-cultured with bone marrow-derived DC (B + DC), and purified mouse primary B cells co-cultured with bone marrow-derived macrophages (B + Mφ). Cells were stimulated with LPS, with or without WGP in vitro for 3 days. (A-C) Single cell suspensions were stained with fluorescein-labeled mAbs. The summarized data are shown for B cells (B220lo CD138+), and immunosuppressive B cells (CD11b+ CD19+). (D) The cytokines of supernatant samples were collected and assayed by LEGENDplex™ MU Th1/Th2 Panel (8-plex). (E) The immunoglobulins of supernatant samples were collected and assayed by LEGENDplex™ Mouse Immunoglobulin Isotyping Panel (6-plex). Calculated the concentration of Ig produced by PCs (B220lo CD138+) (105). A representative histogram from three independent experiments with similar results is shown. (• LPS, ▪ LPS + WGP, * P < 0.05, **P < 0.01, ***P < 0.001)