Fig. 3
From: β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1
β-Glucan regulates B cell immune response stimulated with OVA. OT-II mice splenic cells were stimulated with OVA, with or without WGP in vitro for 72 h. (A) Surface marker expression of B cells was assessed by flow cytometry. (B) Single cell suspensions were stained with fluorescein-labeled mAbs. The summarized data are shown for cells (B220lo CD138+), (CD19+ FAS+ GL7+), and (CD3+ CD4+ CD44+ CXCR5+ PD1+). (C) The cytokines of supernatant samples were collected and assayed by LEGENDplex™ MU Th1/Th2 Panel (8-plex). (D) The immunoglobulins of supernatant samples were collected and assayed by LEGENDplex™ Mouse Immunoglobulin Isotyping Panel (6-plex). Calculated the concentration of Ig produced by PCs (B220lo CD138+) (105). A representative histogram from three independent experiments with similar results is shown. (CON: black, OVA: blue, OVA + WGP: prunosus. * P < 0.05, **P < 0.01, ***P < 0.001)