Fig. 2
From: β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1
β-Glucan regulates B cell immune response stimulated with LPS. Wild type mice splenic cells were stimulated with LPS, WGP, or a combination of both in vitro for 72 h. (A) Surface marker expression of B cells was assessed by flow cytometry. (B) Single cell suspensions were stained with fluorescein-labeled mAbs. The summarized data are shown for cells (B220lo CD138+), (CD19+ FAS+ GL7+), and (CD3+ CD4+ CD44+ CXCR5+ PD1+). (C) The cytokines of supernatant samples were collected and assayed by LEGENDplex™ MU Th1/Th2 Panel (8-plex). (D) The immunoglobulins of supernatant samples were collected and assayed by LEGENDplex™ Mouse Immunoglobulin Isotyping Panel (6-plex). Calculated the concentration of Ig produced by PCs (B220lo CD138+) (105). A representative histogram from three independent experiments with similar results is shown. (CON: black, WGP: yellow, LPS: blue, LPS + WGP: red. * P < 0.05, **P < 0.01, ***P < 0.001)