Fig. 5

Immunofluorescence staining of MPO⁺ and Ly-6G⁺ in the liver samples from each group of mice. A MPO⁺ (red fluorescence) and Ly-6G⁺ (green fluorescence) expression levels in the liver cells of the sham, CLP, anti-IL-6, anti-PD-1, and anti-IL-6/PD-1 mice groups were analyzed using double immunofluorescence staining. In addition, the nuclei were stained using DAPI (blue fluorescence). Merged double-positive cells (MPO⁺ Ly-6G⁺) and nuclei stained using DAPI were combined in the final panel (MPO⁺ + Ly-6G⁺ + DAPI). B Quantitative analysis of Ly6G-positive neutrophil count per field of view. C Quantitative analysis of MPO-positive cell count per field of view. Data are expressed as median and interquartile range; *p < 0.05, **p < 0.005, ***p < 0.001 as determined by one-way ANOVA with post-hoc Tukey’s multiple comparison test. Scale bar: 50 μm