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Fig. 1 | BMC Immunology

Fig. 1

From: Identification of the key targets for sepsis-associated acute kidney injury by RNA sequencing combined with bioinformatics methods

Fig. 1

Research flowchart. RNA sequencing and differential analyses were conducted on peripheral blood samples of 10 healthy participants and 22 septic patients. Meanwhile, the top 1000 kidney-related genes were screened from the online GTEx tool. Subsequently, the intersection of differentially expressed genes (DEGs) and renal damage-associated genes was identified, followed by GO and KEGG analyses on these intersection genes. The prognostic potential of the hub genes was assessed through survival analysis. Through a meta-analysis, we determined the differential expression patterns of hub genes between the sepsis surviving group and the dead group. Then, receiver operator characteristic (ROC) curves were generated to further screen hub genes and clarify their diagnostic value. A single-cell RNA sequencing analysis was the final to elucidate the localization of hub genes in cell lines

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BMC Immunology

ISSN: 1471-2172

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