Fig. 3

Inflammatory response induced by P. gingivalis in diabetic mice. (A and B) The BMDMs of WT and diabetic mice were infected with P. gingivalis for 24 h. (A) Western blot analysis was performed to determine the expression of proteins. Densitometry of the band intensity was performed. (B) IL-1β levels in the cell supernatant were determined using ELISA. (C) Alveolar bone loss was measured using micro-CT in WT and diabetic mice. Representative images (left) and the areas between the cemento-enamel junction and alveolar bone crest of the three molars were calculated using ImageJ and were presented as the area of root exposure in the bar graph (right). *P < 0.050, **P < 0.010, and ***P < 0.001. #p < 0.05 WT-P. gingivalis infection versus DB-P. gingivalis infection