Fig. 1

Purity of Treg cells in relation to the disease categorization. Dot plots of the gating strategy for magnetically purified Treg cells are shown in a. Purified CD4⁺CD25⁺ CD127^dim/− Treg cells were gated based on forward and side scatter. CD3⁺ CD4⁺ T cells were selected from live cells and then analyzed for co-expression of CD127^Low CD25⁺. CD3⁺ CD4⁺ CD127^Low CD25⁺ T cells were more than 95% pure and highly expressed FoxP3 marker (> 70%). The number of FoxP3+ Treg cells was stratified by the participants’ immunological (b) and virological (c) statuses. No significant differences (p > 0.05) were found in the number of FoxP3⁺ Treg cells across all the subgroups after purification. Horizontal bars represent the median and statistical difference was calculated using Kruskal-Wallis test