Fig. 1

Effects of sepsis on the distribution of NK cells and ILC1. a: Typical flow cytometric plot of Sham and CLP splenic NK cells. The cells were pre-gated as CD45⁺CD3⁻. b: The percentage of NK cells in spleen lymphocytes in sham group and CLP group (n = 5; *: p < 0.05). c: The absolute number of spleen NK cells in the sham and CLP group (n = 5; **, p < 0.01). d: The percentage of NK1.1⁺NKp46⁺ cells within liver lymphocytes was assessed. A representative flow cytometric plot is presented on the left, while the summary data are displayed on the right (n = 5; ns: p>0.05). e-f: Representative and summary data of liver NK cells (e) and ILC1 (f), and the relative proportion of NK cells and ILC1 within liver NKp46⁺ cells were generated by flow cytometry. (n = 5; ns: p>0.05; *: p < 0.05;**: p < 0.01). g: Cells were gated for CD45⁺CD3⁻NK1.1⁺NKp46⁺, and then the proportion of liver NK cells and ILC1 were measured based on the expression of CD49a and CD49b (n = 5; **: p < 0.01)