Table 2 The multi-epitope vaccine construct was designed using predicted LBL, CTL, and HTL epitopes from pathogens, together with their matching MHC Class I alleles and immunogenic properties

S. typhimurium

B-cell

LRQELLLKNS

–-

C₅₃H₉₆N₁₆O₁₆

0.14

Non-toxin

1

2

1213.44

8.75

-0.450

156

QPILPKAGDT

–-

C₄₆H₇₈N₁₂O₁₅

0.59

Non-toxin

1

1

1039.20

5.84

-0.510

88

ANVYSSVQAR

–-

C₄₆H₇₅N₁₅O₁₆

0.95

Non-toxin

0

1

1094.19

8.79

-0.240

78

VIPALLAAAT

–-

C₄₄H₇₈N₁₀O₁₂

0.50

Non-toxin

0

0

939.16

5.49

2.120

186

CTL

RPSQRHGSKY

HLA-A*01:01

C₅₁H₈₂N₂₀O₁₅

1.33

Non-toxin

0

3

1215.34

11.0

-2.450

0.0

TQDENPVVHF

HLA-A*02:01

C₅₂H₇₆N₁₄O₁₈

0.33

Non-toxin

0

2

1185.26

4.35

-0.830

58

NMYKDSHHPA

HLA-A*02:01

C₅₁H₇₄N₁₆O₁₆S₁

0.16

Non-toxin

1

1

1199.31

6.92

-1.730

10

TMDHARHGFL

HLA-A*02:01

C₅₁H₇₇N₁₇O₁₄S₁

0.04

Non-toxin

1

1

1184.34

6.61

-0.520

49

HTL

SLQIRLILAVLVALG

HLA-DRB1*01:01

C₇₄H₁₃₅N₁₉O₁₈

1.02

Non-toxin

0

1

1579.00

9.47

2.053

234

ARYIATAVPVKS

HLA-DPA1*02:01/DPB1*14:01

C₅₈H₉₈N₁₆O₁₆

0.41

Non-toxin

0

2

1275.51

9.99

0.458

105

KILAAVIPALLA

HLA-DPA1*02:01/DPB1*14:01

C₅₈H₁₀₅N₁₃O₁₃

0.71

Non-toxin

0

1

1192.55

8.75

2.192

220

FYFISNGPRELL

HLA-DRB3*02:02

C₇₀H₁₀₂N₁₆O₁₈

0.09

Non-toxin

1

1

1455.68

6.0

0.175

97

E. coli

B-cell

SVGKYSASVK

–-

C₄₅H₇₆N₁₂O₁₅

0.81

Non-toxin

0

2

1025.17

9.70

-0.170

68

FRLVDSNGSV

–-

C₄₇H₇₆N₁₄O₁₆

1.40

Non-toxin

1

1

1093.20

5.84

0.150

97

PPPEPVVEPE

–-

C₅₀H₇₆N₁₀O₁₇

0.49

Non-toxin

3

0

1089.21

3.67

-1.010

58

GFINNNGPTH

–-

C₄₆H₆₇N₁₅O₁₅

-0.24

Non-toxin

0

0

1070.13

6.74

-0.950

39

WSLWGNASNT

–-

C₅₁H₇₀N₁₄O₁₆

0.90

Non-toxin

0

0

1135.20

5.52

-0.590

49

CTL

KLVFQEGVSA

HLA-A*02:01

C₄₉H₈₀N₁₂O₁₅

0.55

Non-toxin

1

1

1077.25

6.0

0.470

107

SIPNTLMAAK

HLA-A*03:01

C₄₅H₈₀N₁₂O₁₄S₁

0.17

Non-toxin

0

1

1045.26

8.47

0.330

98

GLLYTSVHQV

HLA-A*02:01

C₅₁H₈₁N₁₃O₁₅

-0.23

Non-toxin

0

0

1116.28

6.74

0.610

136

WRADTKSNVY

HLA-B*27:05

C₅₅H₈₂N₁₆O₁₇

0.34

Non-toxin

1

2

1239.35

8.59

-1.310

39

HTDRRTTLSL

HLA-B*07:02

C₄₉H₈₆N₁₈O₁₇

0.68

Non-toxin

1

2

1199.33

9.61

-1.100

78

HTL

LVFSYAQPFKKY

HLA-DRB5*01:01

C₇₅H₁₀₇N₁₅O₁₇

0.018

Non-toxin

0

2

1490.77

9.53

-0.075

65

NGFFRLVDSNGS

HLA-DRB1*04:05

C₅₇H₈₅N₁₇O₁₉

0.69

Non-toxin

1

1

1312.40

5.84

-0.317

56

ILFKINGTTEIQ

HLA-DRB3*02:02

C₆₃H₁₀₅N₁₅O₁₉

0.71

Non-toxin

1

1

1376.62

6.0

0.325

130

SPVFAGGVEYAI

HLA-DQA1*05:01/DQB1*03:01

C₅₇H₈₄N₁₂O₁₇

0.61

Non-toxin

1

0

1209.36

4.0

0.942

97

MKLLKTVPAIVM

HLA-DRB1*01:01

C₆₂H₁₁₄N₁₄O₁₄S₂

0.49

Non-toxin

0

2

1343.79

10

1.333

154