Fig. 2

Bioinformatics analysis of RNA-seq data. (A)Data distribution after conversion: 20,717 genes in 32 samples. 14,875 were converted to Ensemble gene IDs in our database. The remaining 491 genes were saved in the data using the original ID.(B) Principal component analysis (PCA) showed that there were differences between our sepsis and SIRS groups. (C) Volcano map of differential genes, the selection criteria for differentially expressed genes were set to ≥ 1.2 and the error discovery rate (FDR) was < 0.05. (D) Wayne diagram, blue represents the sepsis differential gene (n = 314), red represents the human mitochondrial gene (n = 1136), and 28 sepsis-related mitochondrial genes were screened