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Fig. 2 | BMC Immunology

Fig. 2

From: Basophil activation in insect venom allergy: comparison of an established test using liquid reagents with a test using 5-color tubes with dried antibody reagents

Fig. 2

Gating strategy of BAT 1. Example of an analysis with the positive control anti-FcɛRI antibody. A Forward Scatter/Side Scatter plot was created to acquire the whole leukocyte population separated into three discrete populations (lymphocytes, monocytes and granulocytes). Plot 2 was created (CCR3-PE vs. Side Scatter) and basophils were gated as CCR3pos and SSClow. Plot 3 was created (CD63-FITC vs. CCR3-PE) to determine stimulated basophils. Activated basophils result in a CD63 positive basophil population (CD63pos/CCR3pos/SSClow) identified in the upper right quadrant

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BMC Immunology

ISSN: 1471-2172

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