Fig. 2
From: PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6
PRMT2 silencing promotes M1 polarization. (A, B) qPCR analysis of Prmt2 mRNA (A) expression and immunoblot analysis of PRMT2 protein (B) in BMDMs transfected with Prmt2 siRNA for 48 h. (C-H) qPCR analysis of Tnf-α (C), Il-1β (D), and Nos2 (E) mRNA expression and FCM analysis of CD86 (F) expression in BMDMs transfected with Prmt2 siRNA for 48 h and then treated with LPS (100 ng/ml) plus IFN-γ (20 ng/ml) for 24 h. (G) Quantification of mean fluorescence intensity of CD86 in (F). (H) Immunoblot analysis of iNOS in BMDMs transfected with Prmt2 siRNA for 48 h and then treated with LPS (100 ng/ml) plus IFN-γ (20 ng/ml) or IL-4 (20 ng/ml) for 24 h. 18s rRNA was used as an endogenous reference for qPCR. Data are representative of three independent experiments (mean ± SD). **p < 0.01, ***p < 0.001, ****p < 0.0001