Fig. 4

Liver, lymph nodes, and brain samples were separated to examine the bacterial presence, and total tissue samples were collected from n = 5 number of guinea pigs from each group, examined with triplicates to avoid false negative/false-positive results in the overall analysis. Each figure displays data from a single biological replicate. (A,B). Showing the AFB + bacilli at 100X magnification during 8th week M.tb infection in guinea pig’s lymph node. (C). Showing the bacilli in liver tissue at 100X magnification during the 8th week M.tb infection