Decreased frequency of Th22 cells and IL-22 cytokine in kidney transplant patients with active cytomegalovirus infection

Table 2 The conditions of the Real-Time-PCR and the sequence of used primers

Real-Time PCR condition
Steps	Temperature	Time	Number of cycles
Initial denaturation	95 ̊C	10 min^a	1x^b
Denaturation Annealing Extension	95 ̊C 60 ̊C 72 ̊C	15 s^c 30 s 30 s	40x
Primers
AHR^d	Forward: 5´AACATCACCTACGCCAGTCG3´ Reverse: 5´TGCCGCTTGGAAGGATTTGA3´
GAPDH^e	Forward: 5´GGACTCATGACCACAGTCC3´ Reverse: 5´CCAGTAGAGGCAGGGATGAT3´

^amin: minute; ^bx: cycle; ^csec: second; ^dAHR: aryl hydrocarbon receptor; ^eGAPDH: glyceraldehyde-3-phosphate dehydrogenase.

ISSN: 1471-2172