Fig. 2

The frequency of Th22 cytokine profile in study groups. A. gating strategy to measure the frequency of Th22 cells (IFN-γ-IL-17 A-IL-22+) and Th17 cells (IFN-γ-IL-17 A+). The green dashes show the frequency of Th17 cells. The IFN-γ-IL-17- double negative cells (red dashes) were gated; then IL-22 + cells were measured. B. The frequency of IL-22 and IL-17 A cytokines in three study groups. C. Comparing the percentage of Th22 cytokine profile (IFN-γ-IL-17 A-IL-22+) in the study groups. D. Comparing the percentage of Th17 cytokine profile (IFN-γ-IL-17 A+) in the study groups. E, F, G. Comparing the percentage of IL-22, IL-17 A, and IFN-γ cytokines in the study groups. SSC, side scatter; FSC, forward scatter; Iso, isotype control; HC, healthy control; HCMV-, kidney transplant patients without active infection; HCMV+, kidney transplant patients with active infection; *, statistically significant at P ≤ 0.05; **, statistically significant at P ≤ 0.01