Fig. 1

The phenotype frequency of Th22 cells in study groups. A. The isolated CD4⁺ T cells against the side and forward scatter to gate on the lymphocyte population. B. The purity analysis of isolated CD4⁺ T cells and gating strategy by flow cytometry. The frequency of ≥ 95% double positive CD3⁺CD4⁺ T cells was considered a pure population. C. Gating strategy to measure the phenotype frequency of Th22 cells (CCR6⁺CCR4⁺CCR10⁺) and Th17 cells (CCR6⁺CCR4⁺). First, the CCR6⁺CCR4⁺ double positive cells (red dashes) were gated for each study group, and then the CCR10⁺ population was measured against side scatter. Red dashes determine the population of Th17 cells (CCR6⁺CCR4⁺). Isotype controls were used to set gates. D. The frequency of each surface chemokine receptor was evaluated against the side scatter in three study groups. Isotype controls were used to set gates. E. The statistical comparison of Th22 cells phenotype frequencies (CCR6⁺CCR4⁺CCR10⁺) between study groups. F. The statistical comparison of Th17 cell phenotype frequencies (CCR6⁺CCR4⁺) between study groups. G, H, I. The statistical comparison of each surface chemokine receptor frequency in different study groups. SSC, side scatter; FSC, forward scatter; Iso, isotype control; HC, healthy control; HCMV-, kidney transplant patients without active infection; HCMV+, kidney transplant patients with active infection; *, statistically significant at P ≤ 0.05