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Table 1 Summary of three manufacturing methods to prepare mature dendritic cell recovery rate

From: Optimizing tumor-associated antigen-stimulated autologous dendritic cell and cytokine-induced killer cell coculture to enhance cytotoxicity for cancer immunotherapy in manufacturing

 

Thaw recovery rate

PBMC→iDC

iDC→mDC

The total recovery rate (mDC)

portocol 1. fresh PBMC > iDC > F-iDC > mDC

83.90%

7.28%

36.76%

2.25% (*)

protocol 2. freeze PBMC > iDC > mDC

60.77%

4.31%

56.43%

1.91%

portocol 3. PBMC > iDC > mDC > F-mDC > mDC

62.00%

4.10%

79.56%

2.14%

  1. We compared three manufacturing methods’ mature dendritic cell (mDC) recovery rates. The lowest mDC recovery rate of 1.91% was obtained with frozen peripheral blood mononuclear cells. The mDC recovery rate from frozen mDCs was 2.14%. The highest recovery rate was 2.25% with cryopreserved immature dendritic cells (iDCs). * indicates p-value < 0.05 compared with the protocol2 on the Student t-test
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BMC Immunology

ISSN: 1471-2172

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