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Fig. 7 | BMC Immunology

Fig. 7

From: TRPA1 activation and Hsp90 inhibition synergistically downregulate macrophage activation and inflammatory responses in vitro

Fig. 7

TRPA1 regulates intracellular calcium influx in LPS-stimulated and Hsp90-inhibited macrophages. RAW 264.7 cells were treated with Fluo-4 AM and assessed via FC for intracellular calcium levels upon combinatorial treatment with either ionomycin, vehicle (DMSO), vehicle + LPS, LPS + 17-AAG, LPS + 17-AAG + HC-030031, and LPS + 17-AAG + AITC. (A) Time-lapse kinetics of intracellular calcium influx. The X-axis of the flow cytometric plots represent approximately 200 s and ‘↓’ represents the addition of reagents/modulators to stimulate cells. (B) Representative line graph depicting fold changes in mean Fluo-4 intensity. The data represent the mean ± SD of three independent experiments.

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BMC Immunology

ISSN: 1471-2172

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