Fig. 5

TRPA1 regulates the Hsp90 inhibition-mediated downregulation of MAPK signaling protein phosphorylation in LPS-stimulated monocytes/macrophages.

RAW 264.7 cells were subjected to different conditions of LPS (500 ng/ml), 17-AAG, HC-030031, and AITC harvested at 15 min and assessed for intracellular signaling proteins p38-MAPK, ERK 1/2, SAPK-JNK and their respective phosphorylated proteins via western blot. (A) Western blot images from the samples represent p38-MAPK, ERK 1/2, SAPK-JNK, and their respective phosphorylated proteins. Bar graph representing the fold change in band intensity of phospho-proteins p-p38-MAPK (B), p-ERK 1/2 (C), and p-SAPK-JNK (D) normalized to the corresponding GAPDH controls. The data represent the mean ± SD of three independent experiments. Differences between groups with a p-value less than 0.05 were considered statistically significant (ns, non-significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001)