Fig. 4

TRPA1 regulates the pro-inflammatory cytokine production in Hsp90-inhibited monocytes/macrophages.

RAW 264.7 or THP-1 cells were subjected to different conditions of LPS/PMA, 17-AAG, HC-030031, or AITC, and the supernatant was collected at 6 and 24 h and assessed for cytokine profile. Bar graph representing IL-6 (A, C, E) and TNF (B, D, F) levels in RAW 264.7 cells stimulated with either LPS (500 ng/ml) (A) / PMA (100 ng/ml) (C) and THP-1 macrophages stimulated with LPS (500ng/ml) (B). The data represent the mean ± SD of three independent experiments. One-way/two-way ANOVA was performed to find statistical significance among groups. Differences between groups with a p-value less than 0.05 were considered statistically significant (ns, non-significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001)