Fig. 5

Effect of CD40L on the memory phenotype of CD8⁺ T cells specific for CMV pp65 antigen. Sorted CD8⁺ T cells were weekly stimulated with irradiated aAPC or aAPC-CD40L loaded with HLA-A*02:01 restricted peptides of pp65 in the presence of IL-2 (10U/mL). After two rounds of stimulation, flow cytometry analysis was done on day 12. (A, B) Effect of CD40L on memory phenotype of CD8⁺ T cells specific for pp65 antigens was analyzed by comparing the CD45RO⁺CD62L⁺ proportion of tetramer positive cells and the CD45RO⁺CCR7⁺ proportion of tetramer positive cells in aAPC stimulated group to aAPC-CD40L stimulated group on day 12 (n = 8). Wilcoxon paired rank test one tailed, *p < 0.05