Fig. 2

CD40L expressed on aAPC enhances the proliferation of CD8⁺ T cells stimulated by anti-CD3 antibody. CD8⁺ T cells from five healthy donors were sorted into memory subsets based on CD45RO and CD62L expression: naïve (CD45RO⁻/CD62L⁺), central memory (CD45RO⁺/CD62L⁺), effector memory (CD45RO⁺/CD62L⁻) and effector CD8⁺ T cells (CD45RO⁻/CD62L⁻). Unsorted total CD8⁺ T cells and sorted CD8⁺ T cell memory subsets were weekly stimulated with irradiated anti-CD3 loaded aAPC or aAPC-CD40L in the presence of IL-2(10U/mL). On day 12, fold expansion of CD8⁺ T cells was analyzed. (A) Representative data of CD45RO and CD62L expression on CD8⁺ T cells. (B) Proportion of each memory subsets of CD8⁺ T cells are shown (n = 5). Bar and error indicate mean and SD. (C) Fold expansions of CD8⁺ T cells stimulated with aAPC were compared to those of CD8⁺ T cells stimulated with aAPC-CD40L on day 12 (n = 5). Wilcoxon paired rank test one tailed, *p < 0.05