Fig. 1

Typical AD-like dermatitis and scratching behavior were induced by DNFB + OVA. A Morphological changes of skin lesion during four weeks. B Skin lesion scorings. The total scores of day 24 and day 28 were compared between D + O group and DNFB group. C Scratching behavior examination. D, E The serum total IgE and OVA-specific IgE in each group. F Serum IgE concentration of acute phase and chronic phase in the DNFB + OVA group. (Date shows mean ± SD, significance levels of data were denoted as *P < 0.05, **P < 0.01, and ***P < 0.001, ****P<0.0001)