Fig. 5

GNT modulated the phenotypic and functional changes of Tregs in vitro via activation of the STAT5 signaling pathway. a Western blot analysis of p-STAT5 protein expression levels in mice with LPS-induced neonatal sepsis treated with or without GNT in vivo. b p-STAT5 protein expression levels in the Tregs, which were isolated from normal mice and then stimulated with LPS and/or GNT in vitro. c p-STAT5 protein expression levels in the Tregs, which were isolated from normal mice and then treated with LPS, GNT, and/or STAT5 inhibitor pimozide or STAT5-IN-1 in vitro. d FOXP3 and CTLA-4 mRNA and protein expression levels in the Tregs, which were isolated from normal mice and then treated with LPS, GNT, and/or STAT5 inhibitor pimozide or STAT5-IN-1 in vitro. Data are expressed as mean ± SD, n = 3. *P < 0.05 versus control group; ^#P < 0.05 versus LPS group; ^$P < 0.05 versus LPS + GNT group