Fig. 1

Study design summarizing the cohort, methodology, and data analysis. 500 μl plasma from subjects in test or validation cohorts were used to isolate EVs using the PureExo kit. EVs were measured by nanoparticle tracking analysis followed by small RNA isolation. Small RNA libraries were prepared and sequenced on Nextseq500, and data was analyzed using the exceRpt pipeline. Candidate miRNAs were validated by qPCR