Fig. 2

CITRUS analyses for the unstimulated cells, clustering on 25 phenotyping markers and comparing median expression of the markers CD371, CD23, CD25, CD28, and HLA-DR between the three groups. a CITRUS trees in which each node denotes different cell clusters. The red nodes illustrate cell populations were the median marker intensities of the respective functional marker differed statistically significantly between the three groups as determined by SAM analyses, FDR 0.05. The parent clusters (the “highest in the hierarchy” significant node) are named based on marker expression shown in C. b Median marker intensities for the various functional markers, shown as box plots (expressing interquartile range (IQR) and median values) and values for each individual within the groups of healthy controls, IgEpos, and IgEneg food allergic subjects, for each parent clusters identified in A. Differences between groups are shown as lines, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001 (Kruskal-Wallis and Dunn’s multiple comparisons test). c CITRUS histograms for the parent clusters identified in A. For each surface marker, the histogram shows the marker expression on cells in the specific cluster (red) against the marker expression on all other cells (blue) – overlapping of histograms will result in purple areas