Fig. 4

The role of RelB in the proliferation and function of Treg. The single bone marrow chimeras were generated with WT or RelB−/− bone marrow cells. 8 weeks later, a the percentage of Treg, Ki67⁺Treg or Ki67⁺CD4⁺Foxp3⁻T cells of WT or RelB^−/− bone marrow chimeric mice were measured by FACS. *** P < 0.001. b The splenic WT or RelB^−/− T cells were sorted by FACS and cultured in vitro. The cell number was measured at 6 h, 24 h and 48 h respectively. c the splentic T cells were sorted and labeled with CFSE, and then these cells were transferred to the sublethal irradiated mice. 3.5 days later, the transferred T cells labeled with CFSE were detected by FACS plot and statistic analysis (d). e CD4⁺CD25⁺ Treg cells from WT or RelB^−/− mice were isolated and mixed with CFSE labeled naïve conventional CD4⁺ T cells (2 × 10⁵) at indicated ratio. 1 μg/ml anti-CD3/CD28 was used to stimulate T cell proliferation. 3 days later, CD4⁺ T cell proliferation was measured by FACS. ** P < 0.01