Fig. 3

RelB controls nTreg but not iTreg development. The bone marrow from 6 weeks old WT or RelB^−/− mice were transferred to the lethal irradiated mice. 8 weeks later, the mice were assayed for splenic Treg frequency and helios expression by FACS (a) and the naive CD4⁺ T cells were sorted by FACS to induce the iTreg with anti-CD3 antibody for 72 h to detect the Foxp3 expression (b). c Treg precursor cells (CD4⁺CD8⁻CD25⁺CD69⁺CD24⁺) into mature Treg cells after IL-2 treatment were detected by FACS