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Fig. 1 | BMC Immunology

Fig. 1

From: Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection

Fig. 1

The dynamic changes of the expression of ABC-transporters and ACAT1 in macrophages response to a BCG infection. Primary bovine alveolar macrophages (AMs) and murine macrophage RAW264.7 cells were infection with BCG at a dose of 10 for indicated time periods, abundances of ABC-transporters and ACAT1 were determined by an immunoblotting (IB) assay. a Representative images of blots for indicated proteins of interest of BCG-infected primary bovine AMs probed with corresponding antibodies (Left panel), and the relative expression of proteins semi-quantified by an optical densitometry analysis (Right panel) demonstrated a dynamic change of protein expression (b) Representative images of blots for indicated proteins of interest of BCG-infected murine macrophage RAW264.7 cells probed with corresponding antibodies (Left panel), and the relative expression of proteins semi-quantified by an optical densitometry analysis (Right panel) demonstrated a dynamic change of protein expression. Both the BCG-infected bovine AMs and RAW264.7 cells exhibited the lowest expression of ABCA1, ACAT1 ABCG1, ABCA5 and ABCA6 at 6, 6, 12, 24 and 24 h post the BCG infection, respectively. Data were expressed as mean ± SEM from three independent experiments. Compared to non-infection controls, *: p < 0.05; **: p < 0.01

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BMC Immunology

ISSN: 1471-2172

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