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Figure 6 | BMC Immunology

Figure 6

From: Interleukin-1beta and tumour necrosis factor-alpha impede neutral lipid turnover in macrophage-derived foam cells

Figure 6

Acyl-CoA activity in triglyceride-loaded THP-1 cells treated with IL-1β. Cells were differentiated, incubated for 24 h with VLDL (50 μg/ml), and then incubated in lipoprotein-free media for an additional 24 h with IL-1β. Cells were homogenized and intracellular fractions isolated by differential centrifugation. Enzymatic activity during a five minutes incubation was assayed in fractions containing mitochondria (open circles) or endoplasmic reticulum (closed circles). Data represents mean ± SEM, n = 6, for a representative experiment repeated three times. * = P < 0.05, ** = P < 0.01.

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BMC Immunology

ISSN: 1471-2172

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