Figure 5
From: Tolerance induced via TLR2 and TLR4 in human dendritic cells: role of IRAK-1
Effect of LPS tolerance induction on levels of IRAK1 protein. Day 7 dendritic cells were cultured for additional 2 days with LPS at 5 ng/ml and then washed and stimulated at 500 ng LPS/ml. Lysates were prepared at 1 h and were subjected to Western blotting for IRAK-1 (A) or SHIP (B). One representative of 4 experiments.