Figure 5

Effects of MgTX or high [K⁺] _e on subsets of CD3⁺ cells. Histograms distribution of oxonol fluorescence in CD3⁺/CD45RO^- and CD3⁺/CD45RO⁺ untreated cells (gray lines) and treated with MgTX (full lines) or high [K⁺]_e (dashed lines). The proportions of CD3⁺/CD45RO^- and CD3⁺/CD45RO⁺ were 30% and 60% (A), 60% and 30% (B) and 45% and 35% (C) for the CD45RO^- and CD45RO⁺ populations of the total CD3⁺, respectively. The black marker indicates depolarized cells as determined by [K⁺]_e treatment. Data are representative of six experiments.