Table 2 Antiendomysial (EmA) and transglutaminase-2 antibody (TG2-ab) secretion to organ culture supernatants after in vitro PT-gliadin challenge. Organ culture of treated celiac disease patient biopsy samples (n = 11) who had small-bowel mucosal IgA deposits. Small-intestinal biopsy samples were cultured either with medium only or with PT-gliadin for 24 or 48 hours. EmA titers were graded according to intensity of staining as follows: negative (neg), weak positive (+) and strong positive (++, +++ or ++++).
EmA | TG2-ab (U) | |||||||
|---|---|---|---|---|---|---|---|---|
Patient | GFD years | Medium 24 h | PT-gliadin24 h | PT-gliadin 48 h | Medium 24 h | PT-gliadin 24 h | PT-gliadin 48 h | |
1 | 1 | ++ | +++ | ++++ | 11.0 | 17.1 | 37.8 | |
2 | 1 | ++ | ++ | +++ | 7.3 | 11.8 | 30.3 | |
3 | 1 | ++ | ++ | +++ | 16.0 | 6.3 | 26.6 | |
4 | 1 | + | ++ | +++ | 12.4 | 13.4 | 9.5 | |
5 | 1 | ++ | neg | ++ | 17.2 | 7.4 | 22.9 | |
6 | 1 | neg | ++ | ++ | 6.5 | 13.1 | 19.9 | |
7 | 1 | neg | neg | + | 7.6 | 5.9 | 24.5 | |
8 | 2 | neg | + | ++ | 7.8 | 5.3 | 14.8 | |
9 | 3 | + | ++ | ++++ | 21.0 | 10.3 | 30.9 | |
10 | 3 | neg | ++ | ++ | 4.9 | 7.0 | nd | |
11 | 4 | ++ | ++ | nd | 24.5 | 11.9 | nd | |
Median | + | ++ | +++* | Median | 11.0 | 10.3 | 24.5† | |