Skip to main content
Figure 6 | BMC Immunology

Figure 6

From: CD11c+ antigen presenting cells from the alveolar space, lung parenchyma and spleen differ in their phenotype and capabilities to activate naïve and antigen-primed T cells

Figure 6

Ex vivo activation of in vivo mycobacterium-primed natural T cells by CD11c+ APCs from BAL, lung parenchyma and spleen. CD11c+ APC populations were isolated from BAL, lung parenchyma and the spleen and pulsed with M.tb CF protein or infected with live mycobacteria. APCs were then co-cultured with CD4+ or CD8+ T cells that were purified from the mice that were infected by live mycobacteria for 17 days (diagram). Cells were co-cultured for 24 h and IFN-γ-secreting T cells were determined by ELISPOT assay. A, IFN-γ-secreting CD4+ T cells. B, IFN-γ-secreting CD8+ T cells. Data are expressed as the mean value ± SD of triplicate samples and representative of two independent experiments. ‡p < 0.05 compared to the corresponding mycobacterial BCG-infected APCs; #p < 0.05 compared to the corresponding spleen data.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us